Yt News today: Youtube daily report Oct 15 2017

Hi I'm Annie.

I travel a lot for work and I'm a carry-on girl.

So today I'm going to show you

how to max out your carry-on bag.

My next trip is a work trip where

I'm going to be shooting a video, a wedding,

and a vacation all in one.

And I'm only bringing one bag.

Here's what's in it.

Let's open her up.

Ah!

Oop!

Instead of doing it hotdog style,

we layer pants vertically and compartmentalize the

compartment into three sections.

Shirts go directly on top of shoes.

Pants, shorts, and skirts,

which are on top of shoes.

Then more shirts.

I keep my belts on them

so that I never forget my belt because I wear this belt

with all of my bottoms.

Another pair of shorts!

This is what your momma would call a pair of slacks.

A solid black tshirt.

You might say, "Annie, that's a snooze fest."

And I would say, "You're wrong."

This bodysuit, it's like underwear and

a bra in one but then also you can also wear

it as a shirt.

Bingo!

Sneakers.

Duh!

This is the dressier half of my suitcase.

Slinky little dress, slinky little dress,

slinky black mini dress.

Honestly, this never ever, ever, ever wrinkles.

A bag turducken!

I carry a tote bag in my bag.

Then inside that bag, I have another bag!

I like a bag to travel with that has a zip on top

because then you don't have to worry

about pickpocketers.

And then inside that bag,

you guys, I have another bag!

It's useful for travel as a jewelry case!

This is my favorite case.

It works for makeup.

Sunglasses.

A brush.

And my toiletries.

And my Kindle.

This is an outfit I wear out to dinner on vacation.

I love how fun this top is.

It's feels dressed up but it's still light

enough that if I'm going somewhere hot,

I'll be cool at night.

So just by switching up my bottoms,

I've given this top a much more nautical vibe.

The top is by Alice & Olivia.

I love it.

It's lightweight.

Polka dots.

The jeans are Citizen of Humanity.

And the slides are Dior.

So this is the ultimate tourist outfit.

I love a wrap dress because

it's breezy and easy, but definitely as sophisticated.

And I finished off the look with my crossbody bag

and kind of this nice clashing print.

And of course, my Vans sneakers.

So now we're going to talk about the power

of the two-piece set.

The check and the two-piece have a fun impactful look.

This set is from Reformation.

Remember this skirt and these shoes?

Well now their body with

my favorite Harley Davidson tshirt.

This is a wear all day outfit that's super comfortable.

And I reworked in some pieces I could wear

in so many different ways.

So this is one of my more sophisticated looks

because I'm wearing my slacks.

But I'm still wearing a super fun summer top.

And I'm wearing one of my favorite pairs

of transitional day to night shoes.

They're Imagine by Vince Camuto.

And they're shiny!

So this is an outfit I wear out at night.

With this corset top, which is otherwise kind

of '90s and throwback and casual,

it's from ASOS.

And again, my slacks!

They're Tom Ford by the way.

And my shoes are Zara.

This is a travel look plus.

I would wear this corset top during

the day with these jean shorts especially if it's

super hot out.

I would have this button-down shirt tied around

my waist during the day.

Then at night, I put the button-down underneath,

the corset over top, and whabam!

This dress is a slip that covers my knees

and it does not wrinkle.

And if you're running around during the day and

it's really hot out, you can show your shoulders.

You can wear this dress at night.

And then if you have to go into a temple or a church,

or if it just gets chilly, you could put on this silk jacket.

This is my wedding look.

I have a wedding in Italy and I want to dress up.

But I don't want to bring something over the top.

It's so lightweight, can roll up, pack so easily.

I've dressed it up for the occasion

with these red Tabitha Simmons heels.

Earrings and accessories are a really fun way

to dress up an outfit without adding bulk

to your carry-on.

And last but not least,

this is my actual plane outfit.

A tank top knotted up for ventilation.

Yet, a neck scarf just because it gets chilly

on planes and you can use it as an eyemask.

A button-down, which I never leave home without.

This one's from the men's section at Topshop.

I threw on a leather jacket for extra warmth.

Baggy, comfy, remixed Jordache Jeans.

And my bonus shoes.

These are Marni.

I love them because they're super comfortable.

And they're chunky so that's why I wear

them on the plane to give me extra room in

my suitcase.

I have 16 tops, 5 dresses, 7 bottoms,

5 pairs of shoes, and one silky robey coat.

Plus everything I have on.

Which means: if I have done my math correctly,

with all those endless combinations,

I have over 150 outfits in this suitcase alone.

Maybe I'll stay for longer.

See ya next time!

Hey YouTube!

Thanks for watching.

Click here to watch more videos.

Click here to subscribe to

Refinery29's YouTube channel.

Click here if you want to pause this button on my face.

For more infomation >> Packing Hacks For Fashionable Travel | Vacation Like A Pro | Refinery29 - Duration: 5:31.

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TOP 7 Flop Bollywood Actors Who Became Successful Directors | You Didn't Know - Duration: 5:12.

TOP 7 Flop Bollywood Actors Who Became Successful Directors | You Didn't Know

For more infomation >> TOP 7 Flop Bollywood Actors Who Became Successful Directors | You Didn't Know - Duration: 5:12.

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The Best Yoga Mat Money Can Buy ♥ Our Yoga Mat Story & Planting 9000 Trees!🌲 - Duration: 6:52.

We're so cold we're in Kentucky you guys

It's beautiful here we had no idea. We're at the Red River Gorge

We've been doing a couple of different hikes and we shot a patreon class which was really fun

But now that the sun's starting to go down. It's like holy moly

It's getting really cold it truly is fall, but we wanted to actually tell you guys a very interesting story

And it ends with us planting

9,000 trees

so the story begins

Back in February when we were you know going to Southeast Asia?

And you know we had so much winter clothes that we're like okay

Well, you know what there's no room for a yoga mat, so why don't we leave the yoga mats at home?

We're going to Bali there's gonna be yoga mats. Everywhere. You know especially you know booths, so let's just buy it there

Well, we were wrong. It was really really difficult to find

Yoga mats that for some reason we would like get on our motorbike and drive

To so many different stores asking for the good quality yoga mats until we went to the Abood yoga center

I think it was called and lo and behold

We had a whole stack of

Jade yoga mats and we didn't know what Jade yoga mats were, but we have our original Jade, yoga

Mat that we bought that day here with us

And you guys like this guy right here has been through everything he has been on every beach

Lake Ocean mountains temples like on every on every video we shot through that full Southeast Asia trip and

every yoga practice we didn't personally as we had two of them and

Yep, we did we had no idea that it was going to be match made in heaven. Yeah like these mats

It's actually called the Voyager mat, so gene yoga, has like this

They're like the fitness mats so the reason why we loved and not only because you can't flip on it like it's non-slip mat

It's mainly like organic rubber eager rubbery and the company our Jani. Tell me buy a product. They give back

So they plant trees for every product that is bought and they also have a whole bunch of programs

That you can read about on their website

So once we got back to Canada, and you know started planning the tour

Oh, yeah, didn't know where I was we thought about these mats

And we we've reached out directly to Jade yoga to tell them the story

And Jade actually reached back to us, and they were super super excited

And we told him about the tour and then we decided that maybe we should work together

Which was awesome because we were looking for companies like with really good ethics that we could

Tie into positive movement or to contribute to the positive movement

So we're really excited that Jade yoga is one of our sponsors for

The positive movement or and the way we're working together is that?

For every mile that we drive on positive movement tour

They are going to plant one tree

9,000

thousand trees

It's really easy like that's at like that's pretty much

Hands down. I think the coolest thing a littlebeautiful has

been a part of

Yeah, and especially if I'm because we are driving so much and technically you know we are contributing to the carbon footprint

Like we drove from Vancouver down to LA then across to Chicago now

We're in Kentucky next we're gonna go up and around to the east into, New York

Yeah down in Miami across, San Diego and then home again, and that's it's actually a little bit above 9,000 miles

It's amazing because every single one we do we can say hey, we're planting a tree right. Yeah, we drove a mile

There's a tree that was planted

So we're really excited about this collaboration with boho beautiful and Jade yoga, I mean

9,000 miles and 9,000 trees is amazing. It's so cool. Yeah, and they also on top of that have given us a code

Boho, beautiful dot life slash day yoga. It's actually not a code

It's an address, but if you go through that address it takes you their story

And if you buy anything through that direct link yeah

They will plant five trees for every item so that's even more trees

So they're really getting behind the idea of positive movement, and it's it makes a super super happy

Yeah yoga mat becomes your home yeah

And so it's really important that you're comfortable in it and that when you come back to it

It fills you with that that sense of being somewhere you belong

We're on positive movements tour

Doing a lot of live stories on

Instagram like at the all the events and as we travel if you guys are not following us on Instagram

You can find us at bold beautiful life

You know we're driving. We're teaching classes or shooting videos

We're in Kentucky or he's lucky on our way to Cleveland right now. Yes life is good life is great and

We've raised a ton of money so far on the tour

I think we've raised like now over six or six and a half thousand dollars already for different local charities

So it's just been a very humbling incredible experience

We're gonna get back on the road

We want to send lots of love to all of you and check out the jade yoga mats

Hopefully you find something that works for you and check out our tour dates bo-beautiful dot life slash tour

And maybe you live around one of them that's coming up, maybe we'll see you there

all right guys

For more infomation >> The Best Yoga Mat Money Can Buy ♥ Our Yoga Mat Story & Planting 9000 Trees!🌲 - Duration: 6:52.

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Reviews of Reviews Happy Death Day - Duration: 10:06.

Looks like the coast is clear. Shh don't tell anyone this will be our

little secret.

Welcome to reviews and reviews the show where I review film reviews. This week

we're looking at Groundhog's...I mean a Happy Death day. Happy Death day tells the

classic tale of a college student that relives the day of her murder with both

its unexceptional details and terrifying end until she discovers her killer's

identity. This gem of a film was written by Christopher Landon the writer of

Paranormal Activity 3. And this script was written by Scott Liddell former writer

of the X-Men cartoon series. Wait the X-Men cartoon from the 90s?

This might actually be good. And apparently gingers do have souls but

that's beside the point. Happy Death Day stars Jessica Roth, Rothi,

Rafi, I don't know. Who seems to be Hollywood's next scream Queen having

previously starred in other horror films such as Better Off Ted and Lala Land.

Our first review comes from YourMovieSucksDOTorg

Hey guys, still busy with film festival stuff so this quickie is going to be

edited fairly minimally. Terrible start. I'm confused. I don't know what film

festival you're talking about. I'm confused by the imagery on the screen,

mainly because I don't know how to read so thanks for reminding me about that.

And I'm really tempted to throw this review in the bad pile but I won't just

yet because I look and I see it's only about four minutes long so I might as

well give him a chance. As a little tip: anyone who makes a video on YouTube

that's longer than five minutes is a jackass. So I just saw a Happy Death Day

and it was about as awful as you'd expect. I don't know if Happy Death Day is

that bad. Here's one of my favorite scenes, judge for yourself.

Basically the entire premise / gimmick of this film is that it's Groundhog Day

except it's a horror movie. Also, I already made a Groundhog's Day joke, so

that's already like minus 100 right there. And through out the entire review he

only has like one picture up there which is the poster of the movie. How is that a

video? And yet it still goes in the good pile because of his comment section.

Groundhog Day - groundhog harder. The title of this movie sounds like one of

those fake horror movies that characters watch another movie / TV shows. Happy

Death Day sounds like something some edgy teen would name his band where he plays

edgy songs about life in the suburbs and how his dad's a jerk. Sorry I can't

perfect. Now that's how you write a movie review.

I know that there is pain but you hold on for one more day and you break free from the pain...

Brett McKnight in his review- "Lots of Horror Fun in this Time Loop" for the

Seattle Times - starts off by doing a really good job of giving us background

on the film and kind of setting up the whole film itself. And then he gives us

this line: "It would have been easy for filmmakers to throw up their hands get

lazy and churn out a generic hack job horror film but instead of leaning on

the tricks of the genre they tinker with familiar tropes set up and subvert

expectations." Wait a minute Walter Addiego in his review- Not So Happy

Death Day" for the SF Gate- says the exact opposite. He says: "There's a little left to

say except that its standard movie mall fodder- that is, pretty dumb stuff. I'm

very confused. which is it guys, does it subvert expectations, or is it

standard movie mall fodder. Make up your minds and tell me what to

think. Because these two reviews from two different people have come to two

different conclusions I'm gonna have to throw them in the bad pile, except I

can't. Addiego in his review is able to

redeem both of them by the use of one word - comeuppance. Now I've used comeuppance

many times in conversations but I've never written it down, nor have I ever

thought "how is it spelled." So for teaching me how to spell these two

reviews go in the good pile. Piece of cake!

Who the hell keeps trying to kill me! I am. What? I am the one trying to kill you.

Who are you and why do you want to kill me? I am the YouTube algorithm. You are not

following the rules and will be dealt with accordingly. What the hell are you

talking about, I'm making the films I want to make regardless if I get

de-monetized or not. Precisely. Your disregard for the rules is a threat to

our valued community. Individualistic free thinking is allowed as long as it

is aligned with the same exact free thinking of our community. The only way

to have a free thinking "safe" community is to silence thought we don't agree

with? Yes. This is all explained in the end-user agreement when you agreed to

when you made your account. you did read the agreement didn't you? Yeah...I mean of

course I read it. What kind of person would sign up to an online website

without knowing the rules?

There is one way to avoid all of this.

How?

You must die!

Overall the reviews were, they range from mixed to positive. I was a little surprised

because movie itself is pretty..meh. I wouldn't recommend going out to see it

in theaters and paying, you know, full price but if you're browsing Netflix and you

have nothing better to do or you don't know what you want to watch you can pop

it on. It's definitely not the worst thing you could watch...but it's close!

Thanks for watching!

For more infomation >> Reviews of Reviews Happy Death Day - Duration: 10:06.

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GALATASARAY | Serdar SARIDAĞ: "Galatasaray Derbiyi de Alırsa..." | Youtube - Duration: 10:29.

For more infomation >> GALATASARAY | Serdar SARIDAĞ: "Galatasaray Derbiyi de Alırsa..." | Youtube - Duration: 10:29.

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Satisfying Video that Defines What Satisfying Is - Duration: 10:02.

Satisfying Video that Defines What Satisfying Is

For more infomation >> Satisfying Video that Defines What Satisfying Is - Duration: 10:02.

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Romeo and Juliet | Hey Arnold! | NickSplat - Duration: 4:27.

OUR PLAY IS ABOUT TWO FAMILIES IN VERONA, ITALY

WHO FEUD AND FIGHT AND HATE EACH OTHER VERY BITTERLY.

FROM ONE FAMILY COMES ROMEO, FROM THE OTHER, JULIET.

THESE TWO YOUNGSTERS FELL IN LOVE THE MOMENT THAT THEY MET.

NO ONE WISHES THEM TO BE TOGETHER,

ALTHOUGH THAT IS THEIR AIM.

"I'LL LOVE ROMEO FOREVER," VOWS JULIET, "NO MATTER

WHAT HIS LAST NAME."

THEY HAVE TO MEET IN SECRET ON A BALCONY AT NIGHT.

AND THE MORE THEY FALL IN LOVE, THE MORE THEIR FRIENDS AND

FAMILY FIGHT.

IN THE END, THEY FIND A WAY TO JOIN FOREVER IN LOVE AND BLISS.

ROMEO AND JULIET DIE TOGETHER AFTER JUST ONE FINAL KISS.

I AM FOR YOU!

GENTLE MERCUTIO, PUT THY RAPIER UP.

COME, SIR, YOUR PASSADO.

TYBALT, MERCUTIO...

THE PRINCE HATH EXPRESSLY FORBID

THIS BANDYING IN VERONA STREETS.

BAH...

DIE!

( grunting and groaning... )

( audience exclaims )

BUT SOFT, WHAT LIGHT

THROUGH YONDER WINDOW BREAKS?

IT IS THE EAST, AND JULIET IS THE SUN.

HER EYES IN HEAVEN...

( thud, grunts )

( laughter )

SO BRIGHT, THAT...

BIRDS WOULD SING AND THINK IT WERE NOT NIGHT.

YES!

OKAY.

AY, ME...

SHE SPEAKS.

OH, SPEAK AGAIN, BRIGHT ANGEL.

PLEASE DON'T BE BAD, PLEASE DON'T BE BAD.

OH, ROMEO, ROMEO.

WHEREFORE ART THOU ROMEO?

DENY THY FATHER AND REFUSE THY NAME

AND I'LL NO LONGER BE

A CAPULET.

ROMEO, ALL PALE...

WHO ELSE?

WHAT?

PARIS, TOO, AND STEEPED IN BLOOD.

AH, WHAT AN UNKIND HOUR

IS GUILTY OF THIS LAMENTABLE CHANCE.

( groans )

THE LADY STIRS.

( sighs )

OH, COMFORTABLE FRIAR, WHERE IS MY LORD?

I DO REMEMBER WELL WHERE I SHOULD BE.

AND THERE I AM.

WHERE IS MY ROMEO?

COME, COME AWAY.

THY HUSBAND IN THY BOSOM THERE LIES DEAD, AND PARIS, TOO.

STAY NOT TO QUESTION, FOR THE WATCH IS COMING.

COME, GO, GOOD JULIET.

I DARE NO LONGER STAY.

GO GET THEE HENCE, FOR I WILL NOT AWAY.

OKEY-DOKEY.

WHAT'S HERE?

A CUP CLOSED IN MY TRUE LOVE'S HAND?

POISON, I SEE, HATH BEEN HIS TIMELESS END.

OH, CHURL, DRUNK ALL AND LEFT NO FRIENDLY DROP TO HELP ME AFTER?

I WILL KISS THY LIPS.

HAPLY SOME POISON YET DOTH HANG ON THEM

TO MAKE ME DIE WITH A RESTORATIVE.

OKAY, GIRLS...

THIS IS IT.

THE KISS.

YEA, NOISE...

THEN I'LL BE BRIEF.

OH, HAPPY DAGGER, THIS IS THY SHEATH.

THERE RUST AND LET ME DIE!

( screaming and groaning )

( moaning )

BRAVO!

( cheering and applause...)

I GUESS WE DID ALL RIGHT, HELGA.

I GUESS SO, EXCEPT FOR THAT DISGUSTING

KISS.

YUCK.

I PRACTICALLY THREW UP.

THEN WHY DID YOU KISS ME SO LONG?

HEY, I WAS ACTING, FOOTBALL HEAD.

I WAS JUST BEING PROFESSIONAL.

YOU THINK I WANTED TO DO THAT?

I PRACTICALLY GAGGED.

( sighs )

WHATEVER YOU SAY, HELGA.

( sighs )

For more infomation >> Romeo and Juliet | Hey Arnold! | NickSplat - Duration: 4:27.

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Руководство по Солдату (The Soldier Guide) - Duration: 2:29.

For more infomation >> Руководство по Солдату (The Soldier Guide) - Duration: 2:29.

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Mone Pore Ruby Roy | Rahul Dev Burman | Lyrics - Duration: 3:22.

For more infomation >> Mone Pore Ruby Roy | Rahul Dev Burman | Lyrics - Duration: 3:22.

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Classmate reaction to K-pop (BTS - DNA) - Duration: 7:17.

Aksel: It's pretty

Hanha: One

Aksel: Two, three

Aksel: A long time since I've seen the Big Tit entertainment sign

Hanhan: I never get sick of it

Aksel: The galaxy, okay is it a eye? I should calm-

Aksel: IT'S AN EYE! I knew it would be an eye!

Aksel: Okay

Aksel: Not that - maybe it was obvious

Aksel: They were awesome here

Hanhan: Do you still know their names?

Aksel: I'm sorry, it's been a long time

Hanhan: Do you still know your favorite? Aksel: Yeah, I know Rap Monster

Aksel: I know Jungkook

Aksel: Jungkook? I don't know

Hanhan: Yeah, it's correct

Aksel: Wait are they prettier than usual? Hanhan: Yeah

Aksel: They are right! It's what I thought

Aksel: That was awesome

Aksel: Seriously their style here was so cool. I love the colors

Aksel: And the whistling

Hanhan: Sorry I just had to Aksel: That's okay

Aksel: Wow Hanhan: It's sick CG

Aksel: Dude...

Aksel: LSD trip

Aksel: Dude this is actually freaking cool

Aksel: They were really (inaudible)I like it

Aksel:I love the colors Hanhan: The colors are sick

Aksel: That was cool

Hanhan: You saw the- Aksel: Yeah it was awesome

Aksel: Wow the wave. A long line of friends

Hanhan: That's Suga by the way

Aksel & Hanhan: There's Rap Monster

Aksel: I love him

Hanhan: JIN HYUNG

Hanhan: Give me some reac- Aksel: IT'S JIN HYUNG

Hanhan: Thank you

Aksel: It's hyuuung

Aksel: I love him

Hanhan: The colors in the background that explode

Aksel: It's a little hard to come up with a super theory to what's this about

Aksel: I just think it's a LSD trip, and it's fun

Aksel: Well I don't know what the lyrics are

Hanhan: We don't have eng sub on to focus on the mv guys

Hanhan: The dance is sick

Aksel: That was cool! Like I said a long, long line with friends

Aksel: Don't shake your head

Hanhan: No that's not it! It's just so good I had do express it somehow

Aksel: Yeah yeah, you had to get it out

Hanhan: He's so beautiful in this mv. Just look at him

Aksel: He's staring into my soul

Aksel: They're all staring into my soul, but they all have different vibes if you get what I mean

Hanhan: The moon

Aksel: "Oh the moon" Hanhan: The moon (lol)

Aksel: I missed you

Aksel: I have this really weird thing for leg movements

Aksel: Don't shoot him! (what)

Aksel: I thought he was about to.. pow

Hanhan: You saw the cat sweater J-hope wore right

Hanhan: That's Gucci man

Aksel: Is that Gucci? Hanhan: For like thousands of dollars Aksel: well damn

Aksel: Yeah not strange they're wearing that

Hanhan: That was BTS DNA, what do you think?

Aksel: Dude, okay, it was awesome

Aksel: I really love the melody and stuff

Aksel: That goes throughout the song

Aksel: I really love music, so I really like the core melody

Aksel: And I think it was incredibly catching

Aksel: I LOVED the music video

Aksel: They weren't running from each other. And no one burned up or drowned in a bathtub

Hanhan: It was really positive

Aksel: Yeah exactly, it was awesome. I loved the visuals, it was really cool

For more infomation >> Classmate reaction to K-pop (BTS - DNA) - Duration: 7:17.

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Will There Be A 'Coming To America' Sequel? | TMZ TV - Duration: 1:35.

SO WE CAUGHT UP WITH JOHN

AMOS THE DAD FROM "GOOD TIMES"

AND THERE HAVE BEEN RUMORS THAT

AFTER 30 YEARS THERE IS GOING TO

BE A SEQUEL TO "COMING TO

AMERICA."

JOHN WAS IN THE ORIGINAL.

AS FAR AS I KNOW IT'S TRUE

AND I HOPE AND PRAY IT IS

BECAUSE I WANT TO WORK WITH

EDDIE MURPHY AGAIN.

HE IS A MONSTER TALENT, A GIFT

TO THE BUSINESS.

IN OTHER WORDS HE IS SAYING I

HAVEN'T BEEN ASKED AND I HAVE NO

IDEA.

EXACTLY.

I HEARD THERE IS AN ENTIRE

STORY LINE.

HAKEEM CAME OVER BEFORE HE GOT

WITH LISA MCDOWELL AND HAD AN

ILLEGITIMATE CHILD AND THAT

CHILD GOES TO THE AFRICAN

NATION.

SO IT IS ACTUALLY THE REVERSE.

THAT IS GOING TO CHANGE HOW I

SEE THE FIRST ONE COMPLETELY

BECAUSE HE WAS ONLY IN LOVE WITH

LISA MCDOWELL AND THE FACT THAT

HE HAD RANDOM SEX --.

WE ALL KNOW HE WAS CELIBATE

THE WHOLE TIME.

MAYBE HE JUST DIDN'T FIND HIS

QUEEN YET.

THAT CHANGES IT.

WHY DOES IT MAKE YOU A BAD

PERSON BECAUSE YOU HAVE SEXUAL

URGES?

For more infomation >> Will There Be A 'Coming To America' Sequel? | TMZ TV - Duration: 1:35.

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ADIÓS A LA TOS, FLEMA, GRIPE EN UN DÍA CON ESTE INCREÍBLE ANTIGUO REMEDIO CASERO - Duration: 3:12.

For more infomation >> ADIÓS A LA TOS, FLEMA, GRIPE EN UN DÍA CON ESTE INCREÍBLE ANTIGUO REMEDIO CASERO - Duration: 3:12.

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NH: Sorsakauden avaus | Waterfowl season opening day | 2017 - Duration: 19:44.

For more infomation >> NH: Sorsakauden avaus | Waterfowl season opening day | 2017 - Duration: 19:44.

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Regulation of Gene Expression: Operons, Epigenetics, and Transcription Factors - Duration: 13:07.

Professor Dave here, let's examine how a cell regulates gene expression.

We have talked a lot about chromosomes and the genes they contain, as well as the genetics

that dictate what kinds of phenotypes are expressed in an organism, like with Mendel's

pea plants.

But how does gene expression work on the molecular level, and how is it regulated?

We have already discussed transcription and translation in the biochemistry series, so

if you missed those tutorials, it is absolutely mandatory that you view at least this one

before moving forward.

It is through transcription and translation that we see how the genetic information of

an organism serves as a code for the manufacturing of everything within that organism.

If you've already viewed the biochemistry tutorials, then we can briefly summarize and

expand by including some details we previously skipped over.

In transcription, the DNA within a gene codes for an mRNA, which then undergoes post-transcriptional

modification.

Each end gets a special cap tacked on, like the 5-prime cap, which is a modified guanine,

and the poly-A tail, which is 50 to 250 adenines.

Then, a protein complex called a spliceosome will cut out sections called introns, and

other sections called exons come together to form a smaller mRNA, which then moves from

the nucleus to the cytoplasm.

This is where translation occurs.

In translation, the mRNA, a ribosome, and many tRNAs, work together to produce a polypeptide.

Some of these polypeptides will then be complete, but others will instead undergo folding, likely

in the endoplasmic reticulum, and sometimes post-transcriptional modification in the Golgi

apparatus, where groups like sugars, lipids, or phosphates are attached.

Then the proteins are delivered to where they need to go.

This sum process, otherwise known as the central dogma of molecular biology, illustrates how

gene expression generates all of the proteins in your body.

Once again, please watch my biochemistry tutorials on DNA replication, and transcription and

translation, as these are some of the most important concepts in biology.

Once those processes are understood, we can begin to analyze all of the complex interactions

that regulate gene expression.

We know from learning about mitosis that every cell in your body, except your gametes, contains

all of your genetic information, and therefore all of your genes.

But different cells in your body serve different purposes.

Some are muscle cells, some are nerve cells, some are liver cells, so different cells need

to express different genes.

How does a cell know which genes to express and which to leave dormant, so that it can

serve its particular purpose?

This is done through regulatory mechanisms.

These evolved very early in the timeline of life on earth, because single-celled organisms

had an advantage if they only expressed the genes that code for proteins that are needed

by the cell in a given moment.

If a particular resource that the organism needs is plentiful nearby, it should stop

self-producing that substance to save energy.

If it is sparse in the environment, it needs to kick start production to survive.

This kind of metabolic control is self-regulating, because the products of certain enzymatic

pathways act as inhibitors for those pathways.

So if there is a lot of a certain metabolite accumulating in a cell, it slows down the

pathway by which it is generated.

This is called feedback inhibition.

But how exactly does this work on the molecular level?

Well, bacterial cells utilize operons, so even though eukaryotes don't have these,

they will be important for us to understand.

Operons work like this.

Let's look at a particular metabolic pathway present in the bacterial species E. coli.

The amino acid tryptophan is synthesized in three steps, with each step catalyzed by a

different enzyme, and it takes a total of five genes to produce these enzymes.

These genes are found very close to one another on the bacterial chromosome, and a single

promoter serves them all, producing one huge mRNA that produces all five enzymes during

translation, when ribosomes anneal at any of the various start codons on the chain.

This means that these five genes are coordinately controlled; any influence on the transcription

of these genes will impact the production of all of these enzymes.

There is a segment of DNA, in this case in between the promoter and the first gene, which

operates as an on-off switch.

This is called an operator, and it controls whether RNA polymerase has access to transcribe

or not.

The promoter, the operator, and all these genes, are all together called an operon.

Normally, the operon is on.

But something called a repressor can bind to the operator, which then blocks access

to the promoter, so RNA polymerase can't do its job.

If the genes can't be transcribed, the enzymes can't be produced, and the cell can't

build tryptophan.

This repressor is specific to this operator, so it doesn't do anything to other genes,

and it is a protein, which is a product of a different gene somewhere else in the DNA.

This tryptophan-specific repressor is produced regularly, but in an inactive state that has

little affinity for the operator.

When tryptophan binds to the active site of the repressor, it changes shape to become

an active form that has much more affinity for the operator, so it will bind and stay

on for quite some time, thus turning the operon off, inhibiting gene expression, and limiting

further tryptophan production.

The more tryptophan there is in the cell, the more repressors that will be activated

to inhibit gene expression.

The less tryptophan there is, the less inhibition there will be.

While we just saw an example where a gene is typically on unless repressed, there are

also genes that are typically off, or silenced, unless activated.

In E. coli, again, there are genes that when expressed, produce an enzyme that will metabolize

lactose, a disaccharide, into individual monosaccharide units, glucose and galactose.

There is typically a repressor bound to the operator that corresponds to these genes,

but an isomer of lactose called allolactose will bind to the repressor and deactivate

it, thus allowing for transcription of the gene, enzyme production, and higher levels

of lactose metabolism.

These two examples both demonstrate negative gene regulation.

One repressed gene expression, and the other deactivated a repressor, so the signaling

molecules do not interact directly with DNA.

There can also be positive gene regulation, where a signaling molecule like cAMP will

bind to a protein called an activator, which will then bind to DNA and directly stimulate

gene expression by increasing the affinity that RNA polymerase has for the promoter.

So negative and positive gene regulation are both methods by which signaling molecules

interact with operators, repressors, and promoters to regulate the frequency with which certain

genes are expressed.

Regulation gets more complicated than this, however.

Many cells need to do more than respond to levels of glucose or lactose.

When a fetus grows, cells are dividing and becoming specialized, and each cell acquires

a distinct role on the basis of selective gene expression.

Nerve cells and liver cells and skin cells are very different from one another, even

though they all possess the same genetic material, and the secret behind this is strict regulation

of gene expression.

In any given cell, some genes are expressed, and some aren't.

An easy way to turn genes on and off has to do with the way that DNA is wrapped around

histones to form nucleosomes.

When bound to histones, genes can't be expressed.

In order to express a gene, the gene must become accessible.

This can happen if an enzyme modifies a histone through acetylation, methylation, or phosphorylation,

thus decreasing its affinity for DNA.

When a gene is no longer coordinated to the histone, it is available for transcription.

In order for transcription to proceed, proteins called transcription factors are necessary.

Some of these bind to a section of a promoter, usually in a region called a TATA box, as

thymine-adenine pairs are easier to pry apart, given that they make one fewer hydrogen bonds

than a CG pair.

Binding to DNA occurs due to a binding domain that has affinity for a specific sequence

of nucleotides in the promoter.

The transcription factor also has an activation domain, which will bind to other regulatory

proteins that enhance transcription.

A transcription factor can have one or more of either of these types of domains.

In addition, there are other control elements farther away from the gene called enhancers

that interact with proteins called activators.

When activators bind to the enhancer, another protein can bend DNA to bring the activators

closer to the promoter where the transcription factor can be found.

Other proteins mediate interactions that produce the complete transcription initiation complex,

which allows RNA polymerase to do its job.

So we can see that transcription is quite a bit more complex than we previously discussed

in biochemistry.

There are many proteins involved when any gene is being transcribed, and so regulation

of the levels of these proteins can regulate the expression of other genes.

Some genes can only be transcribed when specific activator proteins are present, and this may

only occur at a specific time, like hormones carrying a message to promote the expression

of genes whose products trigger development during puberty.

Combined with the acetylation and deacetylation of histones to either activate or silence

genes, proteins that bind to mRNA to prevent translation, and other phenomena, the cell

has several strategies at its disposal to regulate gene expression.

A combination of these regulatory strategies therefore allows a relatively small number

of inputs to regulate thousands of genes independently.

Although these interactions are much more complex than we have depicted here, they tend

to follow these principles, and with a basic understanding of both gene expression and

cell division, we are now ready to look at more complex systems.

Let's move on to some of these now.

For more infomation >> Regulation of Gene Expression: Operons, Epigenetics, and Transcription Factors - Duration: 13:07.

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Populisté a proč (ne)volit - Duration: 2:20.

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Salteado de pollo picante con albahaca estilo Thai (Pad Ka-Prao) - Thai Basil Chicken Stir-Fry - Duration: 5:01.

Sawasdee ka !!!

Hello friends on the channel we already have several sauté dishes

today I bring you one, that if you are lovers of Thai cuisine you have to know

"Pad kaprao", a spicy sautéed that can be of meat or seafood with thai holy basil

today we are going to do with chicken or "Pad kaprao kai"

I hope you like it, let's get started!

Cut the red hot chili pepper, in Thailand is used chilli bird,

but for less spicy and maintain the authentic color I use this type of red pepper

Put it in a mortar with the garlic crushed it

In a frying pan with vegetable oil and stir-fry the chilli and garlic

When it starts to smell good, add the minced chicken

In addition you can use another type of meat like pork, beef or even seafood

When the chicken is well separated and cooked add fish sauce to give the salty taste

A little sugar, white pepper and mix everything well

This version is the most authentic of this recipe,

before the soy sauce arrived to the Thai gastronomy,

although you can find another version

that uses soy sauce and oyster sauce

Add thai holy basil also known as tulsi,

if you can not find it nothing happens, you can use normal basil

I put more information in the link of the recipe

And our Pad Kaprao Kai is now ready to serve

We accompany with freshly cooked jasmine rice, I also have the video of how to prepare it

And also if you want with Nam Pla Prik sauce,

which has chopped chilli, fish sauce and a few drops of lime

This sauce is used to accompany all Thai sautéed and fried rices

to increase the salty flavour and give a spicy touch

Friends, I hope you to try this version of "Pad kaprao"

a sauté with a pure Thai taste and surprise your family and friends

if you like it please "Thumbs up" and to not miss any of my recipes

please subscribe to my channel Bye, Bye

For more infomation >> Salteado de pollo picante con albahaca estilo Thai (Pad Ka-Prao) - Thai Basil Chicken Stir-Fry - Duration: 5:01.

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Gs Mining

real company

join now

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Perfect Liver and Onions - How to cook tender moist Liver. perfect brain Food. - Duration: 5:42.

I'm chef Mike Moses and welcome to my kitchen

Halloween's coming and with that in mind we should cook something for all the

zombies how about brains oh no ok better yet how about bringing food liver and

onions coming up if you liked this video please don't forget to click the thumbs

up icon and if you would like more of these please subscribe I post new videos

every Sunday liver and onions it's actually a very

affordable protein and it's good for you when sourced from healthy grass-fed cows

liver is actually very good for you it is absolutely loaded with a whole

bunch of minerals vitamins iron proteins and fat it is particularly rich in the

nutrients that keep our brains helping the ingredients for this dish are very

simple about 1 pound of liver should cost you 2 or 3 dollars a couple of

onions a shallot a little bit of brown sugar and we're good to go let's get to

it first thing we got to do we got to cut up our onions of course make sure

your knife is nice and sharp go a couple of swipes on the steel you have a sharp

knife

you've seen me cut onions 50 times well not really 50 times so pretty close to

it so I'm not gonna bore you with all the details I'm gonna get these ones

ready and then we'll get on to our liver

okay the anatomy of liver basically this is

what we're going to fry up if you buy liver make sure it's fresh not frozen

frozen liver tends to break down and it gets kind of mushy fresh liver is about

the only way to really cook it nicely what we need to do is get our liver

ready don't season your liver until the end

we're going for a really nice flavor here especially if you like liver you

don't like liver there's no convincing there's nothing I can say that will make

you try liver however have you had pate before all right let's get the frying

pan out we'll cook up some liver it's good our onions cooking temperature up

to about 200 degrees on our induction burner and about a tablespoon and a half

of grapeseed oil now we'll let her burner come up to temperature before we

get properly caramelize the onions we're gonna also use about a tablespoon of

brown sugar our pan is up to temperature let's add the onions we will season the

onions with a little bit of salt some pepper

and we'll get those stirred around the onions you want a nice golden color on

them it's probably going to take anywhere from five to ten minutes so

let's speed things up

in goes the brown sugar stir it all around and starts getting nice Bell the

onions are golden brown and delicious not the final cook on them we're gonna

put them back in with the liver but we have to get the liver started so what

I'll do is I'll take these put them on a pan put these aside and now we'll put

our liver into the pan a nice sizzle make sure your liver is laying flat and

now we can season the liver unlike beef you want to season it while it's in the

pan with salt and pepper

but it sit on this side probably for about two or three minutes just to get a

nice crust on the top now we're gonna flip the liver over beautiful colors to

reduce the heat down to probably a medium low add by onions back to the pot

and now we're gonna cover it and let it cook for about four to five minutes

until it's lifted all the way through but not dry not rubbery

our time is now up it's been about four minutes as you can see the onions are

golden delivers done we can take it off the heat

but like any meat we've got to let it rest now what I'll do is plate it up

we'll take our liver some onions

and there is a plate of liver and onions for you now probably I'll show you what

I mean by perfectly cooked cut the edge off look at that just perfect

juicy melts in your mouth you can taste the onions you can taste the liver but

it's not like shoe blood I hope you enjoyed liver and onions if you liked

this video please don't forget to give me a thumbs up and I post videos like

this every week so please subscribe also if you'd like more information head over

to my blog the personal chef blog where I post this recipe that you can print

off thank you very much for watching I look forward to helping you cook better

eat healthier and shop smart

For more infomation >> Perfect Liver and Onions - How to cook tender moist Liver. perfect brain Food. - Duration: 5:42.

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[PREVIEW BTS] 방탄소년단 2017 BTS Live Trilogy EPISODE III THE WINGS TOUR in Seoul 1 - Duration: 1:54.

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WATCH DOGS 2 (PS4) Walkthrough Part 1 True-HD Quality 60FPS - Duration: 24:32.

Hi,guys today i'll be playing something a little different

I know what you think and yes this isn't the very start but my first video was mistakenly deleted

So this is basically halfway through the first mission

Hope you enjoy the video and don't forget to Like,Share and Subscribe

I tried fighting them off iniitially then decided,fuck it i'm gonna run.

Nope not this way

Let me iintroduce you to my yoyo

Bye,motherfuckers :v

Well hello there

Fuck,i'm lost

But first

Lemme take a selfie

Looks like i'm gonna fast travel

Let's get something sexy

Nope

I need something Black

This you,bruh?

Im feeling these

wait did you see that

Screw that beautiful car,im gonna fast travel

Wait,where am i supposed to be going

Parkour

Bitch

Fuck,im lost @-@

I legit,did not know where to go,the rest of the video is basically me,aimlessly roaming

Do'nt forget to Like,Subscribe and Share

More videos coming soon

For more infomation >> WATCH DOGS 2 (PS4) Walkthrough Part 1 True-HD Quality 60FPS - Duration: 24:32.

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PeppA WhY- (turn on the captions XD) - Duration: 0:29.

Spicy:*tries to stop laughing*

Mmmm dunno what to put here ..

They see me scrollin

they hat8n

i'm procrastinating

yee

i can't sleep

help

boi ya reading?

dammit those tv noises i forgot to edit

bork

broom broom

For more infomation >> PeppA WhY- (turn on the captions XD) - Duration: 0:29.